Measurement

Part:BBa_K2406055

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)


Lox-Term-Lox Measurement Construct

Lox-Term-Lox construct inserted in BBa_J04450 part. If Cre recombinase is present it will cause recombination between the two Lox sites, excising the terminator and allowing expression of the RFP protein. Therefore, this plasmid can be used as an assay for determining levels of Cre recombinase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None